![]() ![]() ![]() (typically quality = max 2 angle = 55 deg). The angle was chosen to include the maximum amount of fetal brain that could be visualized, but kept as narrow as possible to shorten scanning time. If a midsagittal plane could be obtained, a fourth volume was obtained starting with this plane. 3D ultrasound, three static volumes were obtained with high to maximum quality (depending on the amount of fetal movement): the firs t starting with the axial transventricular plane, the second starting with the axial transcerebellar plane, and the third starting with the midcoronal plane. Transabdominal neurosonography was performed with the 2D (2.6-7.7MHz) and 3D (4.0 -8.5MHz) abdominal probes. Biometry (including fetal head circumference, abdominal circumference and femur length) was performed, followed by neurosonography if the biometry conformed to the gestational age. Ultrasound examinations were carried out at two weekly intervals with a General Electric Voluson 730 Expert (General Electric Healthcare, London). The study was approved by the local Medical Ethics Committee of the University Medical Centre, Utrecht, The Netherlands. Women were excluded if there was a discrepancy of seven days or more between the gestational age according to the CRL and the menstrual dating, or if there were risk factors which might influence fetal growth or development, such as maternal disease or previous intra-uterine growth restriction. The gestational (postmenstrual) age was determined by the time from the first day of the last menstruation and confirmed with a crown rump length (CRL) measurement in the first trimester. Twenty eight healthy pregnant women were included after a screening ultrasound examination at 20 weeks had demonstrated a singleton pregnancy with no abnormal findings. 3D ultrasound volumes obtained for PhD study of Lou Pistorius from 2006 – 2008 (files are saved in the data format. ![]()
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